<i>Escherichia coli</i> Extract-Based Cell-Free Expression System as an Alternative for Difficult-to-Obtain Protein Biosynthesis

<i>Escherichia coli</i> Extract-Based Cell-Free Expression System as an Alternative for Difficult-to-Obtain Protein Biosynthesis

Before utilization in biomedical diagnosis, therapeutic treatment, and biotechnology, the diverse variety of peptides and proteins must be preliminarily purified and thoroughly characterized. The recombinant DNA technology and heterologous protein expression have helped simplify the isolation of tar...

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Bibliographic Details
Main Authors: Sviatlana Smolskaya, Yulia A. Logashina, Yaroslav A. Andreev
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:International Journal of Molecular Sciences
Subjects:
recombinant expression
cell-free (cf) protein synthesis
membrane proteins
disulfide bond
protein folding
noncanonical amino acids (naas)
post-translational modification (ptm)
Online Access:https://www.mdpi.com/1422-0067/21/3/928

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https://www.mdpi.com/1422-0067/21/3/928

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