Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays

Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays

SARS-CoV-2 enters cells using its Spike protein, which is also the main target of neutralizing antibodies. Therefore, assays to measure how antibodies and sera affect Spike-mediated viral infection are important for studying immunity. Because SARS-CoV-2 is a biosafety-level-3 virus, one way to simpl...

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Bibliographic Details
Main Authors: Katharine H.D. Crawford, Rachel Eguia, Adam S. Dingens, Andrea N. Loes, Keara D. Malone, Caitlin R. Wolf, Helen Y. Chu, M. Alejandra Tortorici, David Veesler, Michael Murphy, Deleah Pettie, Neil P. King, Alejandro B. Balazs, Jesse D. Bloom
Format: Article
Language:English
Published: MDPI AG 2020-05-01
Series:Viruses
Subjects:
SARS-CoV-2
COVID-19
coronavirus
neutralization assay
lentiviral pseudotype
Spike
Online Access:https://www.mdpi.com/1999-4915/12/5/513

Internet

https://www.mdpi.com/1999-4915/12/5/513

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