Analysis of protein-DNA interactions in chromatin by UV induced cross-linking and mass spectrometry

Analysis of protein-DNA interactions in chromatin by UV induced cross-linking and mass spectrometry

Cross-linking mass spectrometry (XLMS) allows mapping of protein-protein and protein-RNA interactions, but the analysis of protein-DNA complexes remains challenging. Here, the authors develop a UV light-based XLMS workflow to determine protein-DNA interfaces in reconstituted chromatin and isolated n...

Full description

Bibliographic Details
Main Authors: Alexandra Stützer, Luisa M. Welp, Monika Raabe, Timo Sachsenberg, Christin Kappert, Alexander Wulf, Andy M. Lau, Stefan-Sebastian David, Aleksandar Chernev, Katharina Kramer, Argyris Politis, Oliver Kohlbacher, Wolfgang Fischle, Henning Urlaub
Format: Article
Language:English
Published: Nature Publishing Group 2020-10-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-19047-7

Internet

https://doi.org/10.1038/s41467-020-19047-7

Similar Items