Optimizing FRET-FLIM Labeling Conditions to Detect Nuclear Protein Interactions at Native Expression Levels in Living Arabidopsis Roots

Optimizing FRET-FLIM Labeling Conditions to Detect Nuclear Protein Interactions at Native Expression Levels in Living Arabidopsis Roots

Protein complex formation has been extensively studied using Förster resonance energy transfer (FRET) measured by Fluorescence Lifetime Imaging Microscopy (FLIM). However, implementing this technology to detect protein interactions in living multicellular organism at single-cell resolution and under...

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Bibliographic Details
Main Authors: Yuchen Long, Yvonne Stahl, Stefanie Weidtkamp-Peters, Wouter Smet, Yujuan Du, Theodorus W. J. Gadella, Joachim Goedhart, Ben Scheres, Ikram Blilou
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-05-01
Series:Frontiers in Plant Science
Subjects:
protein complexes
protein-protein interaction
fluorescent proteins
in vivo FRET-FLIM
SHORT-ROOT
SCARECROW
Online Access:http://journal.frontiersin.org/article/10.3389/fpls.2018.00639/full

Internet

http://journal.frontiersin.org/article/10.3389/fpls.2018.00639/full

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