Biochemical characterization of mutants in the active site residues of the β-galactosidase enzyme of Bacillus circulans ATCC 31382

Biochemical characterization of mutants in the active site residues of the β-galactosidase enzyme of Bacillus circulans ATCC 31382

The Bacillus circulans ATCC 31382 β-galactosidase (BgaD) is a retaining-type glycosidase of glycoside hydrolase family 2 (GH2). Its commercial enzyme preparation, Biolacta N5, is used for commercial-scale production of galacto-oligosaccharides (GOS). The BgaD active site and catalytic amino acid res...

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Bibliographic Details
Main Authors: Jelle B. Bultema, Bas J.H. Kuipers, Lubbert Dijkhuizen
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:FEBS Open Bio
Subjects:
β-Galactosidase
Galacto-oligosaccharides
Bacillus circulans
Catalytic residue
Mutagenesis
Transglycosylation activity
Online Access:http://www.sciencedirect.com/science/article/pii/S2211546314001089

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http://www.sciencedirect.com/science/article/pii/S2211546314001089

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