Trypsin-induced proteome alteration during cell subculture in mammalian cells

Trypsin-induced proteome alteration during cell subculture in mammalian cells

<p>Abstract</p> <p>Background</p> <p>It is essential to subculture the cells once cultured cells reach confluence. For this, trypsin is frequently applied to dissociate adhesive cells from the substratum. However, due to the proteolytic activity of trypsin, cell surface...

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Bibliographic Details
Main Authors: Lin Cheng-Wen, Lin Szu-Ting, Lu Ying-Chieh, Wu Chieh-Lin, Lyu Ping-Chiang, Chan Hsin-Tsu, Lee Tian-Ren, Chen Yi-Wen, Lai Tzu-Chia, Hsing Hsiang-Wei, Huang Hsiang-Ling, Lai Chih-Ho, Chang Hao-Teng, Chou Hsiu-Chuan, Chan Hong-Lin
Format: Article
Language:English
Published: BMC 2010-05-01
Series:Journal of Biomedical Science
Online Access:http://www.jbiomedsci.com/content/17/1/36

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http://www.jbiomedsci.com/content/17/1/36

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