Laminin-511-derived recombinant fragment and Rho kinase inhibitor Y-27632 facilitate serial cultivation of keratinocytes differentiated from human embryonic stem cells

Introduction: Keratinocytes derived from pluripotent stem cells have a short proliferative lifespan under conventional culture conditions that are optimized for keratinocytes. Recently, a Rho kinase inhibitor, Y-27632, had been used as a standard supplement for culture medium in which the proliferat...

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Bibliographic Details
Main Authors: Ryo Takagi, Akiko Tanuma-Takahashi, Saeko Akiyama, Wakana Kaneko, Chika Miura, Masayuki Yamato, Tatsuya Shimizu, Akihiro Umezawa
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:Regenerative Therapy
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2352320421000572