A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics.
Poxviruses include medically important human pathogens, yet little is known about the specific cellular factors essential for their replication. To identify genes essential for poxvirus infection, we used high-throughput RNA interference to screen the Drosophila kinome for factors required for vacci...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2010-06-01
|
Series: | PLoS Pathogens |
Online Access: | http://europepmc.org/articles/PMC2887478?pdf=render |
id |
doaj-89081ac3ea3f494799c1a5f08f1bacec |
---|---|
record_format |
Article |
spelling |
doaj-89081ac3ea3f494799c1a5f08f1bacec2020-11-25T02:38:52ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742010-06-0166e100095410.1371/journal.ppat.1000954A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics.Theresa S MoserRussell G JonesCraig B ThompsonCarolyn B CoyneSara CherryPoxviruses include medically important human pathogens, yet little is known about the specific cellular factors essential for their replication. To identify genes essential for poxvirus infection, we used high-throughput RNA interference to screen the Drosophila kinome for factors required for vaccinia infection. We identified seven genes including the three subunits of AMPK as promoting vaccinia infection. AMPK not only facilitated infection in insect cells, but also in mammalian cells. Moreover, we found that AMPK is required for macropinocytosis, a major endocytic entry pathway for vaccinia. Furthermore, we show that AMPK contributes to other virus-independent actin-dependent processes including lamellipodia formation and wound healing, independent of the known AMPK activators LKB1 and CaMKK. Therefore, AMPK plays a highly conserved role in poxvirus infection and actin dynamics independent of its role as an energy regulator.http://europepmc.org/articles/PMC2887478?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Theresa S Moser Russell G Jones Craig B Thompson Carolyn B Coyne Sara Cherry |
spellingShingle |
Theresa S Moser Russell G Jones Craig B Thompson Carolyn B Coyne Sara Cherry A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics. PLoS Pathogens |
author_facet |
Theresa S Moser Russell G Jones Craig B Thompson Carolyn B Coyne Sara Cherry |
author_sort |
Theresa S Moser |
title |
A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics. |
title_short |
A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics. |
title_full |
A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics. |
title_fullStr |
A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics. |
title_full_unstemmed |
A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics. |
title_sort |
kinome rnai screen identified ampk as promoting poxvirus entry through the control of actin dynamics. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Pathogens |
issn |
1553-7366 1553-7374 |
publishDate |
2010-06-01 |
description |
Poxviruses include medically important human pathogens, yet little is known about the specific cellular factors essential for their replication. To identify genes essential for poxvirus infection, we used high-throughput RNA interference to screen the Drosophila kinome for factors required for vaccinia infection. We identified seven genes including the three subunits of AMPK as promoting vaccinia infection. AMPK not only facilitated infection in insect cells, but also in mammalian cells. Moreover, we found that AMPK is required for macropinocytosis, a major endocytic entry pathway for vaccinia. Furthermore, we show that AMPK contributes to other virus-independent actin-dependent processes including lamellipodia formation and wound healing, independent of the known AMPK activators LKB1 and CaMKK. Therefore, AMPK plays a highly conserved role in poxvirus infection and actin dynamics independent of its role as an energy regulator. |
url |
http://europepmc.org/articles/PMC2887478?pdf=render |
work_keys_str_mv |
AT theresasmoser akinomernaiscreenidentifiedampkaspromotingpoxvirusentrythroughthecontrolofactindynamics AT russellgjones akinomernaiscreenidentifiedampkaspromotingpoxvirusentrythroughthecontrolofactindynamics AT craigbthompson akinomernaiscreenidentifiedampkaspromotingpoxvirusentrythroughthecontrolofactindynamics AT carolynbcoyne akinomernaiscreenidentifiedampkaspromotingpoxvirusentrythroughthecontrolofactindynamics AT saracherry akinomernaiscreenidentifiedampkaspromotingpoxvirusentrythroughthecontrolofactindynamics AT theresasmoser kinomernaiscreenidentifiedampkaspromotingpoxvirusentrythroughthecontrolofactindynamics AT russellgjones kinomernaiscreenidentifiedampkaspromotingpoxvirusentrythroughthecontrolofactindynamics AT craigbthompson kinomernaiscreenidentifiedampkaspromotingpoxvirusentrythroughthecontrolofactindynamics AT carolynbcoyne kinomernaiscreenidentifiedampkaspromotingpoxvirusentrythroughthecontrolofactindynamics AT saracherry kinomernaiscreenidentifiedampkaspromotingpoxvirusentrythroughthecontrolofactindynamics |
_version_ |
1724789249475084288 |