Stable and transient transformation, and a promoter assay in the selective lignin-degrading fungus, Ceriporiopsis subvermispora

Stable and transient transformation, and a promoter assay in the selective lignin-degrading fungus, Ceriporiopsis subvermispora

Abstract A genetic transformation system was developed for the selective white rot basidiomycete Ceriporiopsis subvermispora using a modified protocol with polyethylene glycol and CaCl2 treatment of the protoplasts and plasmids harboring recombinant hygromycin phosphotransferase (hph) driven by a ho...

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Bibliographic Details
Main Authors: Yoichi Honda, Eiji Tanigawa, Takahisa Tsukihara, Dong Xuan Nguyen, Harunori Kawabe, Naofumi Sakatoku, Junko Watari, Hideaki Sato, Shigekazu Yano, Takashi Tachiki, Toshikazu Irie, Takahito Watanabe, Takashi Watanabe
Format: Article
Language:English
Published: SpringerOpen 2019-06-01
Series:AMB Express
Subjects:
Basidiomycete
Genetic transformation
Transient gene expression
Promoter assay
TATAA sequence
Online Access:http://link.springer.com/article/10.1186/s13568-019-0818-1

Internet

http://link.springer.com/article/10.1186/s13568-019-0818-1

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