Can-Seq: a PCR and DNA sequencing strategy for identifying new alleles of known and candidate genes

Can-Seq: a PCR and DNA sequencing strategy for identifying new alleles of known and candidate genes

Abstract Background Forward genetic screens are a powerful approach for identifying the genes contributing to a trait of interest. However, mutants arising in genes already known can obscure the identification of new genes contributing to the trait. Here, we describe a strategy called Candidate gene...

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Bibliographic Details
Main Authors: Jiangling Cao, Nial R. Gursanscky, Stephen J. Fletcher, Anne Sawyer, Mehershad Wadia, Lachlan McKeough, Marek Coleman, Uwe Dressel, Christelle Taochy, Neena Mitter, Hervé Vaucheret, Bernard J. Carroll
Format: Article
Language:English
Published: BMC 2020-02-01
Series:Plant Methods
Subjects:
Forward genetics
Candidate gene-Sequencing (Can-Seq)
Post-transcriptional gene silencing (PTGS)
Root-to-shoot transmission of PTGS (RTP)
Map-based gene cloning
RNA interference (RNAi)
Online Access:https://doi.org/10.1186/s13007-020-0555-0

Internet

https://doi.org/10.1186/s13007-020-0555-0

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