Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts

Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts

Abstract Background The thermotolerant methylotrophic yeast Ogataea polymorpha has been regarded as an important organism for basic research and biotechnological applications. It is generally recognized as an efficient and safe cell factory in fermentative productions of chemicals, biofuels and othe...

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Bibliographic Details
Main Authors: Laiyou Wang, Aihua Deng, Yun Zhang, Shuwen Liu, Yong Liang, Hua Bai, Di Cui, Qidi Qiu, Xiuling Shang, Zhao Yang, Xiuping He, Tingyi Wen
Format: Article
Language:English
Published: BMC 2018-10-01
Series:Biotechnology for Biofuels
Subjects:
CRISPR–Cas9-assisted multiplex genome editing
Markerless multi-locus integration
Markerless multi-copy integration
Ogataea polymorpha
Saccharomyces cerevisiae
Online Access:http://link.springer.com/article/10.1186/s13068-018-1271-0

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http://link.springer.com/article/10.1186/s13068-018-1271-0

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