Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells
Several proteins are expressed at too low abundance in the Escherichia coli (E. coli) proteome to be detected by standard methods. Here, the authors create a microfluidics-based platform enabling single-molecule counting of low-abundance proteins by mechanically slowing-down their diffusion in live...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2016-05-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms11641 |