Extraction optimization of antioxidant polysaccharides from leaves of Gynura bicolor (Roxb. & Willd.) DC
Orthogonal design was employed to study the effect of extraction time, temperature and liquid-to-solid ratio on the production of antioxidant polysaccharides from leaves of Gynura bicolor (PLG). Analysis of variance was performed on the data obtained. The most relevant variable was extraction time....
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos
2014-06-01
|
Series: | Food Science and Technology |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612014000200027&lng=en&tlng=en |
id |
doaj-8a2a389f8b804509acd546af5919ad13 |
---|---|
record_format |
Article |
spelling |
doaj-8a2a389f8b804509acd546af5919ad132020-11-25T01:27:41ZengSociedade Brasileira de Ciência e Tecnologia de AlimentosFood Science and Technology1678-457X2014-06-0134240240710.1590/fst.2014.0055S0101-20612014000200027Extraction optimization of antioxidant polysaccharides from leaves of Gynura bicolor (Roxb. & Willd.) DCFen Yan0Xuena Yang1Chang Liu2Shangrong Huang3Lan Liao4Caili Fu5Fuzhou UniversityFuzhou UniversityFuzhou UniversityFuzhou UniversityFuzhou UniversityFuzhou UniversityOrthogonal design was employed to study the effect of extraction time, temperature and liquid-to-solid ratio on the production of antioxidant polysaccharides from leaves of Gynura bicolor (PLG). Analysis of variance was performed on the data obtained. The most relevant variable was extraction time. A liquid-solid ratio of 30:1 (v/w), a temperature of 80 °C and an extraction time of 3 h were found to be optimal for PLG. The optimal extraction yield of 4.9% was obtained through additional verification test. Hydroxyl radical-scavenging activity, reducing power and ferrous ion chelating ability of PLG were determined. PLG possess concentration-dependent antioxidant potency and IC50 of PLG was 4.67, 0.24 and 4.31 mg/mL for hydroxyl radical-scavenging and ferric ion chelating abilities as well as reducing power, respectively. The results suggest that G. bicolor polysaccharides could be potential source of natural antioxidant and be contributor to the health benefits of G. bicolor.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612014000200027&lng=en&tlng=enGynura bicolorpolysaccharideorthogonal designantioxidant activity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fen Yan Xuena Yang Chang Liu Shangrong Huang Lan Liao Caili Fu |
spellingShingle |
Fen Yan Xuena Yang Chang Liu Shangrong Huang Lan Liao Caili Fu Extraction optimization of antioxidant polysaccharides from leaves of Gynura bicolor (Roxb. & Willd.) DC Food Science and Technology Gynura bicolor polysaccharide orthogonal design antioxidant activity |
author_facet |
Fen Yan Xuena Yang Chang Liu Shangrong Huang Lan Liao Caili Fu |
author_sort |
Fen Yan |
title |
Extraction optimization of antioxidant polysaccharides from leaves of Gynura bicolor (Roxb. & Willd.) DC |
title_short |
Extraction optimization of antioxidant polysaccharides from leaves of Gynura bicolor (Roxb. & Willd.) DC |
title_full |
Extraction optimization of antioxidant polysaccharides from leaves of Gynura bicolor (Roxb. & Willd.) DC |
title_fullStr |
Extraction optimization of antioxidant polysaccharides from leaves of Gynura bicolor (Roxb. & Willd.) DC |
title_full_unstemmed |
Extraction optimization of antioxidant polysaccharides from leaves of Gynura bicolor (Roxb. & Willd.) DC |
title_sort |
extraction optimization of antioxidant polysaccharides from leaves of gynura bicolor (roxb. & willd.) dc |
publisher |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
series |
Food Science and Technology |
issn |
1678-457X |
publishDate |
2014-06-01 |
description |
Orthogonal design was employed to study the effect of extraction time, temperature and liquid-to-solid ratio on the production of antioxidant polysaccharides from leaves of Gynura bicolor (PLG). Analysis of variance was performed on the data obtained. The most relevant variable was extraction time. A liquid-solid ratio of 30:1 (v/w), a temperature of 80 °C and an extraction time of 3 h were found to be optimal for PLG. The optimal extraction yield of 4.9% was obtained through additional verification test. Hydroxyl radical-scavenging activity, reducing power and ferrous ion chelating ability of PLG were determined. PLG possess concentration-dependent antioxidant potency and IC50 of PLG was 4.67, 0.24 and 4.31 mg/mL for hydroxyl radical-scavenging and ferric ion chelating abilities as well as reducing power, respectively. The results suggest that G. bicolor polysaccharides could be potential source of natural antioxidant and be contributor to the health benefits of G. bicolor. |
topic |
Gynura bicolor polysaccharide orthogonal design antioxidant activity |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612014000200027&lng=en&tlng=en |
work_keys_str_mv |
AT fenyan extractionoptimizationofantioxidantpolysaccharidesfromleavesofgynurabicolorroxbwillddc AT xuenayang extractionoptimizationofantioxidantpolysaccharidesfromleavesofgynurabicolorroxbwillddc AT changliu extractionoptimizationofantioxidantpolysaccharidesfromleavesofgynurabicolorroxbwillddc AT shangronghuang extractionoptimizationofantioxidantpolysaccharidesfromleavesofgynurabicolorroxbwillddc AT lanliao extractionoptimizationofantioxidantpolysaccharidesfromleavesofgynurabicolorroxbwillddc AT cailifu extractionoptimizationofantioxidantpolysaccharidesfromleavesofgynurabicolorroxbwillddc |
_version_ |
1725103806588387328 |