Method for Rapid Analysis of Mutant RNA Polymerase Activity on Templates Containing Unnatural Nucleotides

Method for Rapid Analysis of Mutant RNA Polymerase Activity on Templates Containing Unnatural Nucleotides

Pairs of unnatural nucleotides are used to expand the genetic code and create artificial DNA or RNA templates. In general, an approach is used to engineer orthogonal systems capable of reading codons comprising artificial nucleotides; however, DNA and RNA polymerases capable of recognizing unnatural...

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Bibliographic Details
Main Authors: Tatiana Egorova, Ekaterina Shuvalova, Sabina Mukba, Alexey Shuvalov, Peter Kolosov, Elena Alkalaeva
Format: Article
Language:English
Published: MDPI AG 2021-05-01
Series:International Journal of Molecular Sciences
Subjects:
unnatural nucleotides
T7 RNAP
cell-free translation
Online Access:https://www.mdpi.com/1422-0067/22/10/5186

Internet

https://www.mdpi.com/1422-0067/22/10/5186

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