Five colour variants of bright luminescent protein for real-time multicolour bioimaging

The use of luminescence, rather than fluorescence, for imaging is advantageous in situations where laser illumination should be avoided. Here the authors make five colour variants of bright luminescent proteins based on FRET between NLuc and fluorescent proteins, and show their utility as Ca2+indica...

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Main Authors:	Kazushi Suzuki, Taichi Kimura, Hajime Shinoda, Guirong Bai, Matthew J. Daniels, Yoshiyuki Arai, Masahiro Nakano, Takeharu Nagai
Format:	Article
Language:	English
Published:	Nature Publishing Group 2016-12-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/ncomms13718

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spelling	doaj-8b1143997ccc40c4b902738f03eeb3a62021-05-11T11:11:27ZengNature Publishing GroupNature Communications2041-17232016-12-017111010.1038/ncomms13718Five colour variants of bright luminescent protein for real-time multicolour bioimagingKazushi Suzuki0Taichi Kimura1Hajime Shinoda2Guirong Bai3Matthew J. Daniels4Yoshiyuki Arai5Masahiro Nakano6Takeharu Nagai7Department of Biotechnology, Graduate School of Engineering, Osaka UniversityDepartment of Biotechnology, School of Engineering, Osaka UniversityDepartment of Biotechnology, Graduate School of Engineering, Osaka UniversityDepartment of Biomolecular Science and Engineering, The Institute of Scientific and Industrial Research, Osaka UniversityDivision of Cardiovascular Medicine, BHF Centre for Regenerative MedicineDepartment of Biotechnology, Graduate School of Engineering, Osaka UniversityDepartment of Biotechnology, Graduate School of Engineering, Osaka UniversityDepartment of Biotechnology, Graduate School of Engineering, Osaka UniversityThe use of luminescence, rather than fluorescence, for imaging is advantageous in situations where laser illumination should be avoided. Here the authors make five colour variants of bright luminescent proteins based on FRET between NLuc and fluorescent proteins, and show their utility as Ca2+indicators.https://doi.org/10.1038/ncomms13718
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Kazushi Suzuki Taichi Kimura Hajime Shinoda Guirong Bai Matthew J. Daniels Yoshiyuki Arai Masahiro Nakano Takeharu Nagai
spellingShingle	Kazushi Suzuki Taichi Kimura Hajime Shinoda Guirong Bai Matthew J. Daniels Yoshiyuki Arai Masahiro Nakano Takeharu Nagai Five colour variants of bright luminescent protein for real-time multicolour bioimaging Nature Communications
author_facet	Kazushi Suzuki Taichi Kimura Hajime Shinoda Guirong Bai Matthew J. Daniels Yoshiyuki Arai Masahiro Nakano Takeharu Nagai
author_sort	Kazushi Suzuki
title	Five colour variants of bright luminescent protein for real-time multicolour bioimaging
title_short	Five colour variants of bright luminescent protein for real-time multicolour bioimaging
title_full	Five colour variants of bright luminescent protein for real-time multicolour bioimaging
title_fullStr	Five colour variants of bright luminescent protein for real-time multicolour bioimaging
title_full_unstemmed	Five colour variants of bright luminescent protein for real-time multicolour bioimaging
title_sort	five colour variants of bright luminescent protein for real-time multicolour bioimaging
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2016-12-01
description	The use of luminescence, rather than fluorescence, for imaging is advantageous in situations where laser illumination should be avoided. Here the authors make five colour variants of bright luminescent proteins based on FRET between NLuc and fluorescent proteins, and show their utility as Ca2+indicators.
url	https://doi.org/10.1038/ncomms13718
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Five colour variants of bright luminescent protein for real-time multicolour bioimaging

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