Small RNA profiling of low biomass samples: identification and removal of contaminants

Small RNA profiling of low biomass samples: identification and removal of contaminants

Abstract Background Sequencing-based analyses of low-biomass samples are known to be prone to misinterpretation due to the potential presence of contaminating molecules derived from laboratory reagents and environments. DNA contamination has been previously reported, yet contamination with RNA is us...

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Bibliographic Details
Main Authors: Anna Heintz-Buschart, Dilmurat Yusuf, Anne Kaysen, Alton Etheridge, Joëlle V. Fritz, Patrick May, Carine de Beaufort, Bimal B. Upadhyaya, Anubrata Ghosal, David J. Galas, Paul Wilmes
Format: Article
Language:English
Published: BMC 2018-05-01
Series:BMC Biology
Subjects:
RNA sequencing
Artefact removal
Exogenous RNA in human blood plasma
Contaminant RNA
Spin columns
Online Access:http://link.springer.com/article/10.1186/s12915-018-0522-7

Internet

http://link.springer.com/article/10.1186/s12915-018-0522-7

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