An asymmetric PCR-based, reliable and rapid single-tube native DNA engineering strategy

An asymmetric PCR-based, reliable and rapid single-tube native DNA engineering strategy

<p>Abstract</p> <p>Background</p> <p>Widely used restriction-dependent cloning methods are labour-intensive and time-consuming, while several types of ligase-independent cloning approaches have inherent limitations. A rapid and reliable method of cloning native DNA sequ...

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Bibliographic Details
Main Authors: Bi Yanzhen, Qiao Xianfeng, Hua Zaidong, Zhang Liping, Liu Ximei, Li Li, Hua Wenjun, Xiao Hongwei, Zhou Jingrong, Wei Qingxin, Zheng Xinmin
Format: Article
Language:English
Published: BMC 2012-07-01
Series:BMC Biotechnology
Subjects:
Asymmetric
Bridge PCR
Intramolecular homologous recombination
Myostatin
Online Access:http://www.biomedcentral.com/1472-6750/12/39

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http://www.biomedcentral.com/1472-6750/12/39

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