Single-cell RNA-seq analysis reveals compartment-specific heterogeneity and plasticity of microglia
Summary: Microglia are ubiquitous central nervous system (CNS)-resident macrophages that maintain homeostasis of neural tissues and protect them from pathogen attacks. Yet, their differentiation in different compartments remains elusive. We performed single-cell RNA-seq to compare microglial subtype...
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doaj-8bbddc2b5b1249169340c1a6f247ca9d2021-03-22T12:51:58ZengElsevieriScience2589-00422021-03-01243102186Single-cell RNA-seq analysis reveals compartment-specific heterogeneity and plasticity of microgliaJunying Zheng0Wenjuan Ru1Jay R. Adolacion2Michael S. Spurgat3Xin Liu4Subo Yuan5Rommel X. Liang6Jianli Dong7Andrew S. Potter8S Steven Potter9Ken Chen10Rui Chen11Navin Varadarajan12Shao-Jun Tang13Department of Neuroscience, Cell Biology, & Anatomy, The University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USADepartment of Neuroscience, Cell Biology, & Anatomy, The University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USADepartment of Chemical & Biomolecular Engineering, University of Houston, Houston, TX 77004, USADepartment of Neuroscience, Cell Biology, & Anatomy, The University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USADepartment of Neuroscience, Cell Biology, & Anatomy, The University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USADepartment of Neuroscience, Cell Biology, & Anatomy, The University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USADepartment of Pathology, The University of Texas Medical Branch, Galveston, TX 77555, USADepartment of Pathology, The University of Texas Medical Branch, Galveston, TX 77555, USADivision of Developmental Biology, Children's Hospital Medical Center, Cincinnati, OH 45229, USADivision of Developmental Biology, Children's Hospital Medical Center, Cincinnati, OH 45229, USADepartment of Bioinformatics and Computational Biology, Division of Quantitative Sciences, The University of Texas MD Anderson Cancer Center, Houston 77030, TX, USADepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston 77030, TX, USADepartment of Chemical & Biomolecular Engineering, University of Houston, Houston, TX 77004, USADepartment of Neuroscience, Cell Biology, & Anatomy, The University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USA; Corresponding authorSummary: Microglia are ubiquitous central nervous system (CNS)-resident macrophages that maintain homeostasis of neural tissues and protect them from pathogen attacks. Yet, their differentiation in different compartments remains elusive. We performed single-cell RNA-seq to compare microglial subtypes in the cortex and the spinal cord. A multi-way comparative analysis was carried out on samples from C57/BL and HIV gp120 transgenic mice at two, four, and eight months of age. The results revealed overlapping but distinct microglial populations in the cortex and the spinal cord. The differential heterogeneity of microglia in these CNS regions was further suggested by their disparity of plasticity in response to life span progression and HIV-1 pathogenic protein gp120. Our findings indicate that microglia in different CNS compartments are adapted to their local environments to fulfill region-specific biological functions.http://www.sciencedirect.com/science/article/pii/S2589004221001541ImmunologyDevelopmental BiologyTranscriptomics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Junying Zheng Wenjuan Ru Jay R. Adolacion Michael S. Spurgat Xin Liu Subo Yuan Rommel X. Liang Jianli Dong Andrew S. Potter S Steven Potter Ken Chen Rui Chen Navin Varadarajan Shao-Jun Tang |
spellingShingle |
Junying Zheng Wenjuan Ru Jay R. Adolacion Michael S. Spurgat Xin Liu Subo Yuan Rommel X. Liang Jianli Dong Andrew S. Potter S Steven Potter Ken Chen Rui Chen Navin Varadarajan Shao-Jun Tang Single-cell RNA-seq analysis reveals compartment-specific heterogeneity and plasticity of microglia iScience Immunology Developmental Biology Transcriptomics |
author_facet |
Junying Zheng Wenjuan Ru Jay R. Adolacion Michael S. Spurgat Xin Liu Subo Yuan Rommel X. Liang Jianli Dong Andrew S. Potter S Steven Potter Ken Chen Rui Chen Navin Varadarajan Shao-Jun Tang |
author_sort |
Junying Zheng |
title |
Single-cell RNA-seq analysis reveals compartment-specific heterogeneity and plasticity of microglia |
title_short |
Single-cell RNA-seq analysis reveals compartment-specific heterogeneity and plasticity of microglia |
title_full |
Single-cell RNA-seq analysis reveals compartment-specific heterogeneity and plasticity of microglia |
title_fullStr |
Single-cell RNA-seq analysis reveals compartment-specific heterogeneity and plasticity of microglia |
title_full_unstemmed |
Single-cell RNA-seq analysis reveals compartment-specific heterogeneity and plasticity of microglia |
title_sort |
single-cell rna-seq analysis reveals compartment-specific heterogeneity and plasticity of microglia |
publisher |
Elsevier |
series |
iScience |
issn |
2589-0042 |
publishDate |
2021-03-01 |
description |
Summary: Microglia are ubiquitous central nervous system (CNS)-resident macrophages that maintain homeostasis of neural tissues and protect them from pathogen attacks. Yet, their differentiation in different compartments remains elusive. We performed single-cell RNA-seq to compare microglial subtypes in the cortex and the spinal cord. A multi-way comparative analysis was carried out on samples from C57/BL and HIV gp120 transgenic mice at two, four, and eight months of age. The results revealed overlapping but distinct microglial populations in the cortex and the spinal cord. The differential heterogeneity of microglia in these CNS regions was further suggested by their disparity of plasticity in response to life span progression and HIV-1 pathogenic protein gp120. Our findings indicate that microglia in different CNS compartments are adapted to their local environments to fulfill region-specific biological functions. |
topic |
Immunology Developmental Biology Transcriptomics |
url |
http://www.sciencedirect.com/science/article/pii/S2589004221001541 |
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