Summary: | Yellow strain <i>Flammulina velutipes</i>, which is known as Jinhua mushroom in Taiwan, has become popular among customers due to its distinct texture that is utterly different from white strain <i>F. velutipes</i>. However, there has been little study on the physicochemical properties, antioxidant activities, and biological functions of yellow strain <i>F. velutipes</i> polysaccharides (FVYs). The specific aims of this study are to evaluate and compare the physicochemical properties, antioxidant activities, and biological functions of FVYs and white strain <i>F. velutipes</i> polysaccharides (FVWs) in order to select the strain appropriate for cosmetic ingredient. The FVYs and FVWs were prepared by fractional precipitation (40%, 60%, and 80%). According to the results, FVY-80 showed the greatest antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC<sub>50</sub> = 2.22 mg/mL) and 2,2’ -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assay (IC<sub>50</sub> = 2.04 mg/mL). None of the fractions exhibited cytotoxicity toward L929 cell under a concentration of 500 <inline-formula> <math display="inline"> <semantics> <mi mathvariant="sans-serif">μ</mi> </semantics> </math> </inline-formula>g/mL. FVY-80 significantly reduced the reactive oxygen species (ROS) content in L929 cell by 55.96%, as compared with the H<sub>2</sub>O<sub>2</sub>-induced L929 cell, according to the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay. In conclusion, we suggest that FVY-80 is the best source for a cosmetics ingredient.
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