Generation of Brachyury-mCherry knock-in reporter human pluripotent stem cell line (SNUe003-A-2) using CRISPR/CAS9 nuclease

Generation of Brachyury-mCherry knock-in reporter human pluripotent stem cell line (SNUe003-A-2) using CRISPR/CAS9 nuclease

Brachyury is an embryonic nuclear transcription factor required for mesoderm formation and differentiation. Here, we introduced an mCherry reporter into the C-terminus of Brachyury in the human pluripotent stem cell line SNUhES3 using the CRISPR/Cas9 nuclease approach. Successful gene editing was ve...

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Bibliographic Details
Main Authors: Juwon Jung, Sunsook Hwang, Hyewon Seol, A-Hyeon Kim, Ha Myoung Lee, Jin Jea Sung, Seung Min Jeong, Young Min Choi, Jong Kwan Jun, Dong-wook Kim, Jiho Jang
Format: Article
Language:English
Published: Elsevier 2021-05-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506121001677

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http://www.sciencedirect.com/science/article/pii/S1873506121001677

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