Comprehensive Profiling of HIV Antibody Evolution
Summary: This study evaluates HIV antibody responses and their evolution during the course of HIV infection. A phage display system is used to characterize antibody binding to >3,300 HIV peptides in 57 adults with early- to late-stage infection. We find that the number of unique epitopes targeted...
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doaj-8c14e7e5d4564c0c94e406008899f3db2020-11-24T22:08:08ZengElsevierCell Reports2211-12472019-04-0127514221433.e4Comprehensive Profiling of HIV Antibody EvolutionSusan H. Eshleman0Oliver Laeyendecker1Kai Kammers2Athena Chen3Mariya V. Sivay4Sanjay Kottapalli5Brandon M. Sie6Tiezheng Yuan7Daniel R. Monaco8Divya Mohan9Daniel Wansley10Tomasz Kula11Charles Morrison12Stephen J. Elledge13Ron Brookmeyer14Ingo Ruczinski15H. Benjamin Larman16Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Corresponding authorLaboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH, Baltimore, MD, USA; Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USADivision of Biostatistics and Bioinformatics, Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University School of Medicine, Baltimore, MD, USADepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USADepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USADepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USADepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USADepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USADepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USADepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USADepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USADivision of Genetics, Department of Medicine, Howard Hughes Medical Institute, Brigham and Women’s Hospital, Department of Genetics, Harvard University Medical School, Boston, MA 02115, USAFHI 360, Clinical and Epidemiologic Sciences, Durham, NC, USADivision of Genetics, Department of Medicine, Howard Hughes Medical Institute, Brigham and Women’s Hospital, Department of Genetics, Harvard University Medical School, Boston, MA 02115, USADepartment of Biostatistics, University of California at Los Angeles, Los Angeles, CA, USADepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USADepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Corresponding authorSummary: This study evaluates HIV antibody responses and their evolution during the course of HIV infection. A phage display system is used to characterize antibody binding to >3,300 HIV peptides in 57 adults with early- to late-stage infection. We find that the number of unique epitopes targeted (“antibody breadth”) increases early in infection and then stabilizes or declines. A decline in antibody breadth 9 months to 2 years after infection is associated with subsequent antiretroviral treatment (ART) initiation, and a faster decline in antibody breadth is associated with a shorter time to ART initiation. We identify 266 peptides with increasing antibody reactivity over time and 43 peptides with decreasing reactivity over time. These data are used to design a prototype four-peptide “serosignature” to predict duration of HIV infection. We also demonstrate that epitope engineering can be used to optimize peptide binding properties for applications such as cross-sectional HIV incidence estimation. : Eshleman et al. quantify antibody binding to >3,300 HIV peptides from early- to late-stage infection using a phage display system (VirScan). Binding diversity (breadth) reaches individual-specific set points; breadth decline is associated with CD4 cell loss. Time-dependent binding specificities are identified, optimized, and used to predict duration of HIV infection. Keywords: antibody response to HIV, antibody profiling, HIV incidence, antibody biomarker, serosignature, immunodominant HIV epitopeshttp://www.sciencedirect.com/science/article/pii/S221112471930436X |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Susan H. Eshleman Oliver Laeyendecker Kai Kammers Athena Chen Mariya V. Sivay Sanjay Kottapalli Brandon M. Sie Tiezheng Yuan Daniel R. Monaco Divya Mohan Daniel Wansley Tomasz Kula Charles Morrison Stephen J. Elledge Ron Brookmeyer Ingo Ruczinski H. Benjamin Larman |
spellingShingle |
Susan H. Eshleman Oliver Laeyendecker Kai Kammers Athena Chen Mariya V. Sivay Sanjay Kottapalli Brandon M. Sie Tiezheng Yuan Daniel R. Monaco Divya Mohan Daniel Wansley Tomasz Kula Charles Morrison Stephen J. Elledge Ron Brookmeyer Ingo Ruczinski H. Benjamin Larman Comprehensive Profiling of HIV Antibody Evolution Cell Reports |
author_facet |
Susan H. Eshleman Oliver Laeyendecker Kai Kammers Athena Chen Mariya V. Sivay Sanjay Kottapalli Brandon M. Sie Tiezheng Yuan Daniel R. Monaco Divya Mohan Daniel Wansley Tomasz Kula Charles Morrison Stephen J. Elledge Ron Brookmeyer Ingo Ruczinski H. Benjamin Larman |
author_sort |
Susan H. Eshleman |
title |
Comprehensive Profiling of HIV Antibody Evolution |
title_short |
Comprehensive Profiling of HIV Antibody Evolution |
title_full |
Comprehensive Profiling of HIV Antibody Evolution |
title_fullStr |
Comprehensive Profiling of HIV Antibody Evolution |
title_full_unstemmed |
Comprehensive Profiling of HIV Antibody Evolution |
title_sort |
comprehensive profiling of hiv antibody evolution |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2019-04-01 |
description |
Summary: This study evaluates HIV antibody responses and their evolution during the course of HIV infection. A phage display system is used to characterize antibody binding to >3,300 HIV peptides in 57 adults with early- to late-stage infection. We find that the number of unique epitopes targeted (“antibody breadth”) increases early in infection and then stabilizes or declines. A decline in antibody breadth 9 months to 2 years after infection is associated with subsequent antiretroviral treatment (ART) initiation, and a faster decline in antibody breadth is associated with a shorter time to ART initiation. We identify 266 peptides with increasing antibody reactivity over time and 43 peptides with decreasing reactivity over time. These data are used to design a prototype four-peptide “serosignature” to predict duration of HIV infection. We also demonstrate that epitope engineering can be used to optimize peptide binding properties for applications such as cross-sectional HIV incidence estimation. : Eshleman et al. quantify antibody binding to >3,300 HIV peptides from early- to late-stage infection using a phage display system (VirScan). Binding diversity (breadth) reaches individual-specific set points; breadth decline is associated with CD4 cell loss. Time-dependent binding specificities are identified, optimized, and used to predict duration of HIV infection. Keywords: antibody response to HIV, antibody profiling, HIV incidence, antibody biomarker, serosignature, immunodominant HIV epitopes |
url |
http://www.sciencedirect.com/science/article/pii/S221112471930436X |
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