Enterococcal PcfF Is a Ribbon-Helix-Helix Protein That Recruits the Relaxase PcfG Through Binding and Bending of the oriT Sequence

Enterococcal PcfF Is a Ribbon-Helix-Helix Protein That Recruits the Relaxase PcfG Through Binding and Bending of the oriT Sequence

The conjugative plasmid pCF10 from Enterococcus faecalis encodes a Type 4 Secretion System required for plasmid transfer. The accessory factor PcfF and relaxase PcfG initiate pCF10 transfer by forming the catalytically active relaxosome at the plasmid’s origin-of-transfer (oriT) sequence. Here, we r...

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Bibliographic Details
Main Authors: Saima Rehman, Yang Grace Li, Andreas Schmitt, Lena Lassinantti, Peter J. Christie, Ronnie P.-A. Berntsson
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-05-01
Series:Frontiers in Microbiology
Subjects:
T4SS
accessory factor
conjugation
relaxosome
X-ray crystallography
protein structural and functional analysis
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.00958/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2019.00958/full

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