Expression, Purification, Crystallization, and X-ray Structural Analysis of CRISPR-Associated Protein Cas6 from Methanocaldococcus jannaschii

Expression, Purification, Crystallization, and X-ray Structural Analysis of CRISPR-Associated Protein Cas6 from Methanocaldococcus jannaschii

The CRISPR-associated protein 6, Cas6 protein, is an endoribonuclease that cleaves precursor CRISPR RNAs within the repeat sequence to release specific invader-targeting RNAs. Cas6 protein can recognize different sequences by their specific scaffold. To investigate its binding mode, we purified and...

Full description

Bibliographic Details
Main Authors: Ming-Chang Lee, Shih-Ting Tseng, Juan-Cheng Yang, Tung-Ju Hsieh, Shang-Chuen Wu, Shu-Min Kuan, Ming-Jen Chen, Ming-Chiu Chang, Chun-Chiu Wang, Hsiu-Lin Chen, Guor-Cheng Fang, Winn-Jung Huang, Tzu-Ping Ko, Yeh Chen
Format: Article
Language:English
Published: MDPI AG 2017-11-01
Series:Crystals
Subjects:
CRISPR
Cas proteins
endoribonuclease
RNA processing
RNAi
Online Access:https://www.mdpi.com/2073-4352/7/11/344

Internet

https://www.mdpi.com/2073-4352/7/11/344

Similar Items