An Optimized Protocol for the Isolation and Functional Analysis of Human Lung Mast Cells

An Optimized Protocol for the Isolation and Functional Analysis of Human Lung Mast Cells

Background: Mast cells are tissue-resident inflammatory cells defined by their high granularity and surface expression of the high-affinity IgE receptor, FcεRI, and CD117/KIT, the receptor for stem cell factor (SCF). There is a considerable heterogeneity among mast cells, both phenotypically and fun...

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Bibliographic Details
Main Authors: Avinash Ravindran, Elin Rönnberg, Joakim S. Dahlin, Luca Mazzurana, Jesper Säfholm, Ann-Charlotte Orre, Mamdoh Al-Ameri, Peter Peachell, Mikael Adner, Sven-Erik Dahlén, Jenny Mjösberg, Gunnar Nilsson
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-10-01
Series:Frontiers in Immunology
Subjects:
mast cells
lung
enzymatic digestion protocols
human mast cells
mast cell isolation
Online Access:https://www.frontiersin.org/article/10.3389/fimmu.2018.02193/full

Internet

https://www.frontiersin.org/article/10.3389/fimmu.2018.02193/full

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