Single-Molecule Studies on a FRET Biosensor: Lessons from a Comparison of Fluorescent Protein Equipped versus Dye-Labeled Species

Single-Molecule Studies on a FRET Biosensor: Lessons from a Comparison of Fluorescent Protein Equipped versus Dye-Labeled Species

Bacterial periplasmic binding proteins (PBPs) undergo a pronounced ligand-induced conformational change which can be employed to monitor ligand concentrations. The most common strategy to take advantage of this conformational change for a biosensor design is to use a Förster resonance energ...

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Bibliographic Details
Main Authors: Henning Höfig, Michele Cerminara, Ilona Ritter, Antonie Schöne, Martina Pohl, Victoria Steffen, Julia Walter, Ignacio Vergara Dal Pont, Alexandros Katranidis, Jörg Fitter
Format: Article
Language:English
Published: MDPI AG 2018-11-01
Series:Molecules
Subjects:
Förster resonance energy transfer (FRET)
single molecule studies
biosensor
fluorescent protein (FP)
conformational change
hinge motion
ligand binding
glucose sensor
Online Access:https://www.mdpi.com/1420-3049/23/12/3105

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https://www.mdpi.com/1420-3049/23/12/3105

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