Single-Molecule Studies on a FRET Biosensor: Lessons from a Comparison of Fluorescent Protein Equipped versus Dye-Labeled Species
Bacterial periplasmic binding proteins (PBPs) undergo a pronounced ligand-induced conformational change which can be employed to monitor ligand concentrations. The most common strategy to take advantage of this conformational change for a biosensor design is to use a Förster resonance energ...
Main Authors: | Henning Höfig, Michele Cerminara, Ilona Ritter, Antonie Schöne, Martina Pohl, Victoria Steffen, Julia Walter, Ignacio Vergara Dal Pont, Alexandros Katranidis, Jörg Fitter |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-11-01
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Series: | Molecules |
Subjects: | |
Online Access: | https://www.mdpi.com/1420-3049/23/12/3105 |
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