Synthetic polycistronic sequences in eukaryotes

The need for co-ordinate, high-level, and stable expression of multiple genes is essential for the engineering of biosynthetic circuits and metabolic pathways. This work outlines the functionality and design of IRES- and 2 A-peptide-based constructs by comparing different strategies for co-expressio...

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Bibliographic Details
Main Authors: Xuekun Wang, Mario Andrea Marchisio
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2021-12-01
Series:Synthetic and Systems Biotechnology
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X21000466