Using RT-qPCR, Proteomics, and Microscopy to Unravel the Spatio-Temporal Expression and Subcellular Localization of Hordoindolines Across Development in Barley Endosperm

Using RT-qPCR, Proteomics, and Microscopy to Unravel the Spatio-Temporal Expression and Subcellular Localization of Hordoindolines Across Development in Barley Endosperm

Hordeum vulgare (barley) hordoindolines (HINs), HINa, HINb1, and HINb2, are orthologous proteins of wheat puroindolines (PINs) that are small, basic, cysteine-rich seed-specific proteins and responsible for grain hardness. Grain hardness is, next to its protein content, a major quality trait. In bar...

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Bibliographic Details
Main Authors: Azita Shabrangy, Valentin Roustan, Siegfried Reipert, Marieluise Weidinger, Pierre-Jean Roustan, Eva Stoger, Wolfram Weckwerth, Verena Ibl
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-06-01
Series:Frontiers in Plant Science
Subjects:
barley endosperm
hordoindoline
spatio-temporal
laser microdissection
label-free shotgun proteomics
RT-qPCR
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2018.00775/full

Internet

https://www.frontiersin.org/article/10.3389/fpls.2018.00775/full

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