Loss of MAR1 Function is a Marker for Co-Selection of CRISPR-Induced Mutations in Plants

In this study, we describe the establishment of the knockout marker gene MAR1 for selection of CRISPR/Cas9-edited Arabidopsis seedlings and tomato explants in tissue culture. MAR1 encodes a transporter that is located in mitochondria and chloroplasts and is involved in iron homeostasis. It also oppo...

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Bibliographic Details
Main Authors: Jannis Rinne, Claus-Peter Witte, Marco Herde
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-08-01
Series:Frontiers in Genome Editing
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2021.723384/full