Magnesium Restores Activity to Peripheral Blood Cells in a Patient With Functionally Impaired Interleukin-2-Inducible T Cell Kinase
Interleukin-2-inducible T cell kinase (ITK) is critical for T cell signaling and cytotoxicity, and control of Epstein-Barr virus (EBV). We identified a patient with a novel homozygous missense mutation (D540N) in a highly conserved residue in the kinase domain of ITK who presented with EBV-positive...
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doaj-8d21737dd3e54543aa8b76ed898800702020-11-25T01:08:14ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-08-011010.3389/fimmu.2019.02000408682Magnesium Restores Activity to Peripheral Blood Cells in a Patient With Functionally Impaired Interleukin-2-Inducible T Cell KinaseMatthew K. Howe0Kennichi Dowdell1Amitava Roy2Julie E. Niemela3Wyndham Wilson4Joshua J. McElwee5Jason D. Hughes6Jeffrey I. Cohen7Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United StatesLaboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United StatesBioinformatics and Computational Biosciences Branch, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, MT, United StatesDepartment of Laboratory Medicine, Clinical Center, NIH, Bethesda, MD, United StatesLymphoid Malignancies Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD, United StatesMerck Research Laboratories, Boston, MA, United StatesMerck Research Laboratories, Boston, MA, United StatesLaboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United StatesInterleukin-2-inducible T cell kinase (ITK) is critical for T cell signaling and cytotoxicity, and control of Epstein-Barr virus (EBV). We identified a patient with a novel homozygous missense mutation (D540N) in a highly conserved residue in the kinase domain of ITK who presented with EBV-positive lymphomatoid granulomatosis. She was treated with interferon and chemotherapy and her disease went into remission; however, she has persistent elevation of EBV DNA in the blood, low CD4 T cells, low NK cells, and nearly absent iNKT cells. Molecular modeling predicts that the mutation increases the flexibility of the ITK kinase domain impairing phosphorylation of the protein. Stimulation of her T cells resulted in reduced phosphorylation of ITK, PLCγ, and PKC. The CD8 T cells were moderately impaired for cytotoxicity and degranulation. Importantly, addition of magnesium to her CD8 T cells in vitro restored cytotoxicity and degranulation to levels similar to controls. Supplemental magnesium in patients with mutations in another protein important for T cell signaling, MAGT1, was reported to restore EBV-specific cytotoxicity. Our findings highlight the critical role of ITK for T cell activation and suggest the potential for supplemental magnesium to treat patients with ITK deficiency.https://www.frontiersin.org/article/10.3389/fimmu.2019.02000/fullIL-2 inducible T cell kinaseITKEpstein-Barr viruslymphomatoid granulomatosismagnesiumimmunodeficiency |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Matthew K. Howe Kennichi Dowdell Amitava Roy Julie E. Niemela Wyndham Wilson Joshua J. McElwee Jason D. Hughes Jeffrey I. Cohen |
spellingShingle |
Matthew K. Howe Kennichi Dowdell Amitava Roy Julie E. Niemela Wyndham Wilson Joshua J. McElwee Jason D. Hughes Jeffrey I. Cohen Magnesium Restores Activity to Peripheral Blood Cells in a Patient With Functionally Impaired Interleukin-2-Inducible T Cell Kinase Frontiers in Immunology IL-2 inducible T cell kinase ITK Epstein-Barr virus lymphomatoid granulomatosis magnesium immunodeficiency |
author_facet |
Matthew K. Howe Kennichi Dowdell Amitava Roy Julie E. Niemela Wyndham Wilson Joshua J. McElwee Jason D. Hughes Jeffrey I. Cohen |
author_sort |
Matthew K. Howe |
title |
Magnesium Restores Activity to Peripheral Blood Cells in a Patient With Functionally Impaired Interleukin-2-Inducible T Cell Kinase |
title_short |
Magnesium Restores Activity to Peripheral Blood Cells in a Patient With Functionally Impaired Interleukin-2-Inducible T Cell Kinase |
title_full |
Magnesium Restores Activity to Peripheral Blood Cells in a Patient With Functionally Impaired Interleukin-2-Inducible T Cell Kinase |
title_fullStr |
Magnesium Restores Activity to Peripheral Blood Cells in a Patient With Functionally Impaired Interleukin-2-Inducible T Cell Kinase |
title_full_unstemmed |
Magnesium Restores Activity to Peripheral Blood Cells in a Patient With Functionally Impaired Interleukin-2-Inducible T Cell Kinase |
title_sort |
magnesium restores activity to peripheral blood cells in a patient with functionally impaired interleukin-2-inducible t cell kinase |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2019-08-01 |
description |
Interleukin-2-inducible T cell kinase (ITK) is critical for T cell signaling and cytotoxicity, and control of Epstein-Barr virus (EBV). We identified a patient with a novel homozygous missense mutation (D540N) in a highly conserved residue in the kinase domain of ITK who presented with EBV-positive lymphomatoid granulomatosis. She was treated with interferon and chemotherapy and her disease went into remission; however, she has persistent elevation of EBV DNA in the blood, low CD4 T cells, low NK cells, and nearly absent iNKT cells. Molecular modeling predicts that the mutation increases the flexibility of the ITK kinase domain impairing phosphorylation of the protein. Stimulation of her T cells resulted in reduced phosphorylation of ITK, PLCγ, and PKC. The CD8 T cells were moderately impaired for cytotoxicity and degranulation. Importantly, addition of magnesium to her CD8 T cells in vitro restored cytotoxicity and degranulation to levels similar to controls. Supplemental magnesium in patients with mutations in another protein important for T cell signaling, MAGT1, was reported to restore EBV-specific cytotoxicity. Our findings highlight the critical role of ITK for T cell activation and suggest the potential for supplemental magnesium to treat patients with ITK deficiency. |
topic |
IL-2 inducible T cell kinase ITK Epstein-Barr virus lymphomatoid granulomatosis magnesium immunodeficiency |
url |
https://www.frontiersin.org/article/10.3389/fimmu.2019.02000/full |
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