Field diagnosis and genotyping of chikungunya virus using a dried reverse transcription loop-mediated isothermal amplification (LAMP) assay and MinION sequencing.

Field diagnosis and genotyping of chikungunya virus using a dried reverse transcription loop-mediated isothermal amplification (LAMP) assay and MinION sequencing.

Detection and sequencing of chikungunya virus (CHIKV) genome was performed using a combination of a modified reverse transcription loop-mediated isothermal amplification (RT-LAMP) method and a MinION sequencer. We developed the protocol for drying all the reagents for the RT-LAMP in a single reactio...

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Bibliographic Details
Main Authors: Kyoko Hayashida, Yasuko Orba, Patricia C Sequeira, Chihiro Sugimoto, William W Hall, Yuki Eshita, Yutaka Suzuki, Lucky Runtuwene, Patricia Brasil, Guilherme Calvet, Cintia D S Rodrigues, Carolina C Dos Santos, Maria A M Mares-Guia, Junya Yamagishi, Ana M B de Filippis, Hirofumi Sawa
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-06-01
Series:PLoS Neglected Tropical Diseases
Online Access:https://doi.org/10.1371/journal.pntd.0007480

Internet

https://doi.org/10.1371/journal.pntd.0007480

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