Human sperm vitrification: the state of the art
Abstract Sperm cryopreservation has been widely used in assisted reproductive technology (ART) and has resulted in millions of live births. Two principal approaches have been adopted: conventional (slow) freezing and vitrification. As a traditional technique, slow freezing has been successfully empl...
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doaj-8d733509a53f438ab2b06ff1405701822020-11-25T02:10:15ZengBMCReproductive Biology and Endocrinology1477-78272020-03-0118111010.1186/s12958-020-00580-5Human sperm vitrification: the state of the artYong Tao0Erika Sanger1Arpornrad Saewu2Marie-Claude Leveille3Ottawa Fertility CenterOttawa Fertility CenterOttawa Fertility CenterOttawa Fertility CenterAbstract Sperm cryopreservation has been widely used in assisted reproductive technology (ART) and has resulted in millions of live births. Two principal approaches have been adopted: conventional (slow) freezing and vitrification. As a traditional technique, slow freezing has been successfully employed and widely used at ART clinics whereas the latter, a process to solidify liquid into an amorphous or glassy state, may become a faster alternative method of sperm cryopreservation with significant benefits in regard to simple equipment and applicability to fertility centers. Sperm vitrification has its own limitations. Firstly, small volume of load is usually plunged to liquid nitrogen to achieve high cooling rate, which makes large volume sample cryopreservation less feasible. Secondly, direct contact with liquid nitrogen increases the potential risk of contamination. Recently, new carriers have been developed to facilitate improved control over the volume and speed, and new strategies have been implemented to minimize the contamination risk. In summary, although sperm vitrification has not yet been applied in routine sperm cryopreservation, its potential as a standard procedure is growing.http://link.springer.com/article/10.1186/s12958-020-00580-5CryopreservationVitrificationContaminationLiquid nitrogenSpermatozoaSemen |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yong Tao Erika Sanger Arpornrad Saewu Marie-Claude Leveille |
spellingShingle |
Yong Tao Erika Sanger Arpornrad Saewu Marie-Claude Leveille Human sperm vitrification: the state of the art Reproductive Biology and Endocrinology Cryopreservation Vitrification Contamination Liquid nitrogen Spermatozoa Semen |
author_facet |
Yong Tao Erika Sanger Arpornrad Saewu Marie-Claude Leveille |
author_sort |
Yong Tao |
title |
Human sperm vitrification: the state of the art |
title_short |
Human sperm vitrification: the state of the art |
title_full |
Human sperm vitrification: the state of the art |
title_fullStr |
Human sperm vitrification: the state of the art |
title_full_unstemmed |
Human sperm vitrification: the state of the art |
title_sort |
human sperm vitrification: the state of the art |
publisher |
BMC |
series |
Reproductive Biology and Endocrinology |
issn |
1477-7827 |
publishDate |
2020-03-01 |
description |
Abstract Sperm cryopreservation has been widely used in assisted reproductive technology (ART) and has resulted in millions of live births. Two principal approaches have been adopted: conventional (slow) freezing and vitrification. As a traditional technique, slow freezing has been successfully employed and widely used at ART clinics whereas the latter, a process to solidify liquid into an amorphous or glassy state, may become a faster alternative method of sperm cryopreservation with significant benefits in regard to simple equipment and applicability to fertility centers. Sperm vitrification has its own limitations. Firstly, small volume of load is usually plunged to liquid nitrogen to achieve high cooling rate, which makes large volume sample cryopreservation less feasible. Secondly, direct contact with liquid nitrogen increases the potential risk of contamination. Recently, new carriers have been developed to facilitate improved control over the volume and speed, and new strategies have been implemented to minimize the contamination risk. In summary, although sperm vitrification has not yet been applied in routine sperm cryopreservation, its potential as a standard procedure is growing. |
topic |
Cryopreservation Vitrification Contamination Liquid nitrogen Spermatozoa Semen |
url |
http://link.springer.com/article/10.1186/s12958-020-00580-5 |
work_keys_str_mv |
AT yongtao humanspermvitrificationthestateoftheart AT erikasanger humanspermvitrificationthestateoftheart AT arpornradsaewu humanspermvitrificationthestateoftheart AT marieclaudeleveille humanspermvitrificationthestateoftheart |
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