Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP

Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP

The popular genetically-encoded Ca2+ indicator, GCaMP, has several side-effects. Here the authors show that GCaMP containing CaM interferes with gating and signaling of L-type calcium channels, which disrupts Ca2+ dynamics and gene expression, and develop GCaMP-X to overcome these limitations.

Bibliographic Details
Main Authors: Yaxiong Yang, Nan Liu, Yuanyuan He, Yuxia Liu, Lin Ge, Linzhi Zou, Sen Song, Wei Xiong, Xiaodong Liu
Format: Article
Language:English
Published: Nature Publishing Group 2018-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-03719-6

Internet

https://doi.org/10.1038/s41467-018-03719-6

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