Design and Testing of Vector-Producing HEK293T Cells Bearing a Genomic Deletion of the SV40 T Antigen Coding Region

Design and Testing of Vector-Producing HEK293T Cells Bearing a Genomic Deletion of the SV40 T Antigen Coding Region

The use of the human embryonic kidney (HEK) 293T cell line to manufacture vectors for in vivo applications raises safety concerns due to the presence of SV40 T antigen-encoding sequences. We used CRISPR-Cas9 genome editing to remove the SV40 T antigen-encoding sequences from HEK293T cells by transfe...

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Bibliographic Details
Main Authors: Dahae Hailey Bae, Michael Marino, Brian Iaffaldano, Sydney Fenstermaker, Sandra Afione, Takele Argaw, Jacob McCright, Anna Kwilas, John A. Chiorini, Andrew E. Timmons, Jakob Reiser
Format: Article
Language:English
Published: Elsevier 2020-09-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
Lentivirus
AAV
vector manufacturing
HEK293T cells
SV40 T antigen
CRISPR/Cas9
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050120301558

Internet

http://www.sciencedirect.com/science/article/pii/S2329050120301558

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