Obtaining the tbf gene which encodes immunodominant epitopes of pathogenic cholera strains

We experimentally carried out the synthesis of the tbf gene that encodes highly-immunogenic epitopes of pathogenic cholera strains, through the cloning of the tbf gene in pGEM-T Easy plasmid. Moreover, we tested the hybrid gene sequence for absence of mutations, using the Sanger sequencing. We also...

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Bibliographic Details
Main Authors: Bazarnova Yulia, Bolotnikova Tatiana, Aronova Ekaterina
Format: Article
Language:English
Published: Sciendo 2019-09-01
Series:Current Issues in Pharmacy and Medical Sciences
Subjects:
Online Access:https://doi.org/10.2478/cipms-2019-0024