Apoptosis induced by proteasome inhibition in human myoblast cultures
Dysfunction of the ubiquitin-proteasome system has recently been implicated in the pathogenesis of some untreatable myodegenerative diseases characterized by the formation of ubiquitinated inclusions in skeletal muscles. We have developed an in vitro model of proteasomal dysfunction by applying inhi...
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2009-06-01
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Series: | European Journal of Histochemistry |
Online Access: | https://www.ejh.it/index.php/ejh/article/view/981 |
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doaj-8dc522c85e7e4ec0bcd9676a8ab45d832020-11-25T01:24:13ZengPAGEPress PublicationsEuropean Journal of Histochemistry 1121-760X2038-83062009-06-0150210.4081/981699Apoptosis induced by proteasome inhibition in human myoblast culturesJ SassoneA CiammolaC TilocaM GlionnaG MeolaE MancinelliV SilaniDysfunction of the ubiquitin-proteasome system has recently been implicated in the pathogenesis of some untreatable myodegenerative diseases characterized by the formation of ubiquitinated inclusions in skeletal muscles. We have developed an in vitro model of proteasomal dysfunction by applying inhibitors of the proteasome to primary adult human skeletal muscle cultures. Our data show that proteasome inhibition causes both cytoplasmic accumulation of ubiquitinated inclusions and apoptotic death, the latter through accumulation of active caspase-3.https://www.ejh.it/index.php/ejh/article/view/981 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
J Sassone A Ciammola C Tiloca M Glionna G Meola E Mancinelli V Silani |
spellingShingle |
J Sassone A Ciammola C Tiloca M Glionna G Meola E Mancinelli V Silani Apoptosis induced by proteasome inhibition in human myoblast cultures European Journal of Histochemistry |
author_facet |
J Sassone A Ciammola C Tiloca M Glionna G Meola E Mancinelli V Silani |
author_sort |
J Sassone |
title |
Apoptosis induced by proteasome inhibition in human myoblast cultures |
title_short |
Apoptosis induced by proteasome inhibition in human myoblast cultures |
title_full |
Apoptosis induced by proteasome inhibition in human myoblast cultures |
title_fullStr |
Apoptosis induced by proteasome inhibition in human myoblast cultures |
title_full_unstemmed |
Apoptosis induced by proteasome inhibition in human myoblast cultures |
title_sort |
apoptosis induced by proteasome inhibition in human myoblast cultures |
publisher |
PAGEPress Publications |
series |
European Journal of Histochemistry |
issn |
1121-760X 2038-8306 |
publishDate |
2009-06-01 |
description |
Dysfunction of the ubiquitin-proteasome system has recently been implicated in the pathogenesis of some untreatable myodegenerative diseases characterized by the formation of ubiquitinated inclusions in skeletal muscles. We have developed an in vitro model of proteasomal dysfunction by applying inhibitors of the proteasome to primary adult human skeletal muscle cultures. Our data show that proteasome inhibition causes both cytoplasmic accumulation of ubiquitinated inclusions and apoptotic death, the latter through accumulation of active caspase-3. |
url |
https://www.ejh.it/index.php/ejh/article/view/981 |
work_keys_str_mv |
AT jsassone apoptosisinducedbyproteasomeinhibitioninhumanmyoblastcultures AT aciammola apoptosisinducedbyproteasomeinhibitioninhumanmyoblastcultures AT ctiloca apoptosisinducedbyproteasomeinhibitioninhumanmyoblastcultures AT mglionna apoptosisinducedbyproteasomeinhibitioninhumanmyoblastcultures AT gmeola apoptosisinducedbyproteasomeinhibitioninhumanmyoblastcultures AT emancinelli apoptosisinducedbyproteasomeinhibitioninhumanmyoblastcultures AT vsilani apoptosisinducedbyproteasomeinhibitioninhumanmyoblastcultures |
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1725118175985532928 |