Apoptosis induced by proteasome inhibition in human myoblast cultures

Apoptosis induced by proteasome inhibition in human myoblast cultures

Dysfunction of the ubiquitin-proteasome system has recently been implicated in the pathogenesis of some untreatable myodegenerative diseases characterized by the formation of ubiquitinated inclusions in skeletal muscles. We have developed an in vitro model of proteasomal dysfunction by applying inhi...

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Main Authors: J Sassone, A Ciammola, C Tiloca, M Glionna, G Meola, E Mancinelli, V Silani
Format: Article
Language:English
Published: PAGEPress Publications 2009-06-01
Series:European Journal of Histochemistry
Online Access:https://www.ejh.it/index.php/ejh/article/view/981
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spelling doaj-8dc522c85e7e4ec0bcd9676a8ab45d832020-11-25T01:24:13ZengPAGEPress PublicationsEuropean Journal of Histochemistry 1121-760X2038-83062009-06-0150210.4081/981699Apoptosis induced by proteasome inhibition in human myoblast culturesJ SassoneA CiammolaC TilocaM GlionnaG MeolaE MancinelliV SilaniDysfunction of the ubiquitin-proteasome system has recently been implicated in the pathogenesis of some untreatable myodegenerative diseases characterized by the formation of ubiquitinated inclusions in skeletal muscles. We have developed an in vitro model of proteasomal dysfunction by applying inhibitors of the proteasome to primary adult human skeletal muscle cultures. Our data show that proteasome inhibition causes both cytoplasmic accumulation of ubiquitinated inclusions and apoptotic death, the latter through accumulation of active caspase-3.https://www.ejh.it/index.php/ejh/article/view/981
collection DOAJ
language English
format Article
sources DOAJ
author J Sassone
A Ciammola
C Tiloca
M Glionna
G Meola
E Mancinelli
V Silani
spellingShingle J Sassone
A Ciammola
C Tiloca
M Glionna
G Meola
E Mancinelli
V Silani
Apoptosis induced by proteasome inhibition in human myoblast cultures
European Journal of Histochemistry
author_facet J Sassone
A Ciammola
C Tiloca
M Glionna
G Meola
E Mancinelli
V Silani
author_sort J Sassone
title Apoptosis induced by proteasome inhibition in human myoblast cultures
title_short Apoptosis induced by proteasome inhibition in human myoblast cultures
title_full Apoptosis induced by proteasome inhibition in human myoblast cultures
title_fullStr Apoptosis induced by proteasome inhibition in human myoblast cultures
title_full_unstemmed Apoptosis induced by proteasome inhibition in human myoblast cultures
title_sort apoptosis induced by proteasome inhibition in human myoblast cultures
publisher PAGEPress Publications
series European Journal of Histochemistry
issn 1121-760X
2038-8306
publishDate 2009-06-01
description Dysfunction of the ubiquitin-proteasome system has recently been implicated in the pathogenesis of some untreatable myodegenerative diseases characterized by the formation of ubiquitinated inclusions in skeletal muscles. We have developed an in vitro model of proteasomal dysfunction by applying inhibitors of the proteasome to primary adult human skeletal muscle cultures. Our data show that proteasome inhibition causes both cytoplasmic accumulation of ubiquitinated inclusions and apoptotic death, the latter through accumulation of active caspase-3.
url https://www.ejh.it/index.php/ejh/article/view/981
work_keys_str_mv AT jsassone apoptosisinducedbyproteasomeinhibitioninhumanmyoblastcultures
AT aciammola apoptosisinducedbyproteasomeinhibitioninhumanmyoblastcultures
AT ctiloca apoptosisinducedbyproteasomeinhibitioninhumanmyoblastcultures
AT mglionna apoptosisinducedbyproteasomeinhibitioninhumanmyoblastcultures
AT gmeola apoptosisinducedbyproteasomeinhibitioninhumanmyoblastcultures
AT emancinelli apoptosisinducedbyproteasomeinhibitioninhumanmyoblastcultures
AT vsilani apoptosisinducedbyproteasomeinhibitioninhumanmyoblastcultures
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