Evaluating and Correcting Inherent Bias of microRNA Expression in Illumina Sequencing Analysis

Evaluating and Correcting Inherent Bias of microRNA Expression in Illumina Sequencing Analysis

microRNA (miRNA) expression profiles based on the highly powerful Illumina sequencing technology rely on the construction of cDNA libraries in which adaptor ligation is known to deeply favor some miRNAs over others. This introduces erroneous measurements of the miRNA abundances and relative miRNA qu...

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Bibliographic Details
Main Authors: Anne Baroin-Tourancheau, Yan Jaszczyszyn, Xavier Benigni, Laurence Amar
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-04-01
Series:Frontiers in Molecular Biosciences
Subjects:
miRNA expression profile
high-throughput sequencing
Illumina technology
ligation bias
miRNA abundance
cerebellum
Online Access:https://www.frontiersin.org/article/10.3389/fmolb.2019.00017/full

Internet

https://www.frontiersin.org/article/10.3389/fmolb.2019.00017/full

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