Streamlining the Pipeline for Generation of Recombinant Affinity Reagents by Integrating the Affinity Maturation Step

Streamlining the Pipeline for Generation of Recombinant Affinity Reagents by Integrating the Affinity Maturation Step

Often when generating recombinant affinity reagents to a target, one singles out an individual binder, constructs a secondary library of variants, and affinity selects a tighter or more specific binder. To enhance the throughput of this general approach, we have developed a more integrated strategy...

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Bibliographic Details
Main Authors: Renhua Huang, Kevin T. Gorman, Chris R. Vinci, Elena Dobrovetsky, Susanne Gräslund, Brian K. Kay
Format: Article
Language:English
Published: MDPI AG 2015-09-01
Series:International Journal of Molecular Sciences
Subjects:
affinity maturation
affinity selection
error-prone PCR
FN3 monobody
loop shuffling
Kunkel mutagenesis
megaprimer
off-rate selection
phage-display
secondary library
Online Access:http://www.mdpi.com/1422-0067/16/10/23587

Internet

http://www.mdpi.com/1422-0067/16/10/23587

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