Hydrophobically Modified siRNAs Silence Huntingtin mRNA in Primary Neurons and Mouse Brain

Hydrophobically Modified siRNAs Silence Huntingtin mRNA in Primary Neurons and Mouse Brain

Applications of RNA interference for neuroscience research have been limited by a lack of simple and efficient methods to deliver oligonucleotides to primary neurons in culture and to the brain. Here, we show that primary neurons rapidly internalize hydrophobically modified siRNAs (hsiRNAs) added di...

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Bibliographic Details
Main Authors: Julia F Alterman, Lauren M Hall, Andrew H Coles, Matthew R Hassler, Marie-Cecile Didiot, Kathryn Chase, Jasmin Abraham, Emily Sottosanti, Emily Johnson, Ellen Sapp, Maire F Osborn, Marian Difiglia, Neil Aronin, Anastasia Khvorova
Format: Article
Language:English
Published: Elsevier 2015-01-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
delivery
Huntington's disease
neurodegenerative disease
RNA interference
small interfering RNA
Online Access:http://www.sciencedirect.com/science/article/pii/S216225311630052X

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http://www.sciencedirect.com/science/article/pii/S216225311630052X

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