Analysis of Transcriptome and Epitranscriptome in Plants Using PacBio Iso-Seq and Nanopore-Based Direct RNA Sequencing

Analysis of Transcriptome and Epitranscriptome in Plants Using PacBio Iso-Seq and Nanopore-Based Direct RNA Sequencing

Nanopore sequencing from Oxford Nanopore Technologies (ONT) and Pacific BioSciences (PacBio) single-molecule real-time (SMRT) long-read isoform sequencing (Iso-Seq) are revolutionizing the way transcriptomes are analyzed. These methods offer many advantages over most widely used high-throughput shor...

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Bibliographic Details
Main Authors: Liangzhen Zhao, Hangxiao Zhang, Markus V. Kohnen, Kasavajhala V. S. K. Prasad, Lianfeng Gu, Anireddy S. N. Reddy
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-03-01
Series:Frontiers in Genetics
Subjects:
SMRT isoform sequencing
nanopore direct RNA sequencing
RNA modification
alternative splicing
alternative polyadenylation
epitranscriptome
Online Access:https://www.frontiersin.org/article/10.3389/fgene.2019.00253/full

Internet

https://www.frontiersin.org/article/10.3389/fgene.2019.00253/full

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