Optimization of PCR-RFLP Method to Confirm the Identification of Enterococcus faecalis and Enterococcus Faecium Isolated from Food Samples

Optimization of PCR-RFLP Method to Confirm the Identification of Enterococcus faecalis and Enterococcus Faecium Isolated from Food Samples

Commercial kits for species identification of Enterococcus faecalis and Enterococcus faecium are in some cases (environmental isolates) incorrect to distinguish this species of enterococci. Also the positive results of multiplex PCR are subjected by amount and purity of genomic DNA. This study aimed...

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Bibliographic Details
Main Authors: Miroslav Kročko, Michal Gábor, Monika Lavová, Martina Miluchová, Margita Čanigová, Jana Bezeková, Viera Ducková, Anna Trakovická
Format: Article
Language:English
Published: Agroprint Timisoara 2011-12-01
Series:Scientific Papers Animal Science and Biotechnologies
Subjects:
pcr-rflp
16s rrna
multiplex pcr
enterococcus faecalis
enterococcus faecium
Online Access:http://spasb.ro/index.php/spasb/article/view/698

Internet

http://spasb.ro/index.php/spasb/article/view/698

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