Improving low-temperature activity of Sulfolobus acidocaldarius 2-keto-3-deoxygluconate aldolase
Sulfolobus acidocaldarius 2-keto-3-deoxygluconate aldolase (SacKdgA) displays optimal activity at 95°C and is studied as a model enzyme for aldol condensation reactions. For application of SacKdgA at lower temperatures, a library of randomly generated mutants was screened for improved synthesis of 2...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2009-01-01
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| Series: | Archaea |
| Online Access: | http://dx.doi.org/10.1155/2009/194186 |