Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification?

In observations by confocal or conventional fluorescence microscopy, important factors should be considered in order to obtain accurate images. One of them, such as the fluorescence bleaching from highest intensity to lowest signal of fluorescence is a common problem with several DNA fluorochromes a...

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Bibliographic Details
Main Authors: CRISTIAN GALLARDO-ESCÁRATE, JOSUÉ ÁLVAREZ-BORREGO, ELISABETH VON BRAND, ENRIQUE DUPRÉ, MIGUEL ÁNGEL DEL RÍO-PORTILLA
Format: Article
Language:English
Published: BMC 2007-01-01
Series:Biological Research
Subjects:
Online Access:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100004&lng=en&tlng=en