Upscaled CTAB-Based DNA Extraction and Real-Time PCR Assays for Fusarium culmorum and F. graminearum DNA in Plant Material with Reduced Sampling Error

Upscaled CTAB-Based DNA Extraction and Real-Time PCR Assays for Fusarium culmorum and F. graminearum DNA in Plant Material with Reduced Sampling Error

Fusarium graminearum Schwabe (Gibberella zeae Schwein. Petch.) and F. culmorum W.G. Smith are major mycotoxin producers in small-grain cereals afflicted with Fusarium head blight (FHB). Real-time PCR (qPCR) is the method of choice for species-specific, quantitative estimation of fungal biomass in pl...

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Bibliographic Details
Main Authors: Petr Karlovsky, Christoph Brandfass
Format: Article
Language:English
Published: MDPI AG 2008-11-01
Series:International Journal of Molecular Sciences
Subjects:
Fusarium graminearum Schwabe
Fusarium culmorum W.G. Smith
realtime PCR
DNA extraction
sampling error
Fusarium head blight
Online Access:http://www.mdpi.com/1422-0067/9/11/2306/

Internet

http://www.mdpi.com/1422-0067/9/11/2306/

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