Probing Intracellular Element Concentration Changes during Neutrophil Extracellular Trap Formation Using Synchrotron Radiation Based X-Ray Fluorescence.

High pressure frozen (HPF), cryo-substituted microtome sections of 2 μm thickness containing human neutrophils (white blood cells) were analyzed using synchrotron radiation based X-ray fluorescence (SR nano-XRF) at a spatial resolution of 50 nm. Besides neutrophils from a control culture, we also an...

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Bibliographic Details
Main Authors: Björn De Samber, Maria J Niemiec, Brecht Laforce, Jan Garrevoet, Eva Vergucht, Riet De Rycke, Peter Cloetens, Constantin F Urban, Laszlo Vincze
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5094720?pdf=render