Label-free 3D visualization of cellular and tissue structures in intact muscle with second and third harmonic generation microscopy.

Second and Third Harmonic Generation (SHG and THG) microscopy is based on optical effects which are induced by specific inherent physical properties of a specimen. As a multi-photon laser scanning approach which is not based on fluorescence it combines the advantages of a label-free technique with r...

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Bibliographic Details
Main Authors: Markus Rehberg, Fritz Krombach, Ulrich Pohl, Steffen Dietzel
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3225396?pdf=render