Microfilariae Trigger Eosinophil Extracellular DNA Traps in a Dectin-1-Dependent Manner
Summary: Eosinophils mediate protection against filarial nematodes. Our results demonstrate that eosinophil extracellular traps (EETosis) are induced by microfilariae and infective L3 larvae of Litomosoides sigmodontis. These extracellular DNA traps inhibit microfilariae motility in a DNA- and conta...
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doaj-8e98423ad80d4e999f2ac7bb48ffd06a2021-01-14T04:17:06ZengElsevierCell Reports2211-12472021-01-01342108621Microfilariae Trigger Eosinophil Extracellular DNA Traps in a Dectin-1-Dependent MannerAlexandra Ehrens0Benjamin Lenz1Anna-Lena Neumann2Samuela Giarrizzo3Julia Jennifer Reichwald4Stefan Julian Frohberger5Wiebke Stamminger6Benedikt Christian Buerfent7Frédéric Fercoq8Coralie Martin9Daniel Kulke10Achim Hoerauf11Marc Peter Hübner12Institute for Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn 53127, GermanyInstitute for Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn 53127, GermanyInstitute for Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn 53127, GermanyInstitute for Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn 53127, GermanyInstitute for Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn 53127, GermanyInstitute for Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn 53127, GermanyInstitute for Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn 53127, GermanyInstitute for Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn 53127, GermanyUMR7245 MCAM Museum National d’Histoire Naturelle, CNRS, Paris, FranceUMR7245 MCAM Museum National d’Histoire Naturelle, CNRS, Paris, FranceElanco Animal Health - Research and Exploratory Development, Monheim 40789, GermanyInstitute for Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn 53127, Germany; German Center for Infection Research (DZIF), partner site Bonn-Cologne, Bonn, GermanyInstitute for Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn 53127, Germany; German Center for Infection Research (DZIF), partner site Bonn-Cologne, Bonn, Germany; Corresponding authorSummary: Eosinophils mediate protection against filarial nematodes. Our results demonstrate that eosinophil extracellular traps (EETosis) are induced by microfilariae and infective L3 larvae of Litomosoides sigmodontis. These extracellular DNA traps inhibit microfilariae motility in a DNA- and contact-dependent manner in vitro. Accordingly, microfilariae-injection triggers DNA release in an eosinophil-dependent manner in vivo and microfilariae covered with DNA traps are cleared more rapidly. Using dectin-1, we identify the required receptor for the microfilariae-induced EETosis, whereas signaling via other C-type lectin receptors, prior priming of eosinophils, and presence of antibodies are not required. The DNA released upon microfilariae-induced EETosis is mainly of mitochondrial origin, but acetylated and citrullinated histones are found within the traps. We further demonstrate that the presented DNA-dependent inhibition of microfilariae motility by eosinophils represents a conserved mechanism, as microfilariae from L. sigmodontis and the canine heartworm Dirofilaria immitis induce ETosis in murine and human eosinophils.http://www.sciencedirect.com/science/article/pii/S2211124720316107filariaextracellular DNA trapseosinophilsmicrofilariaL3 larvaETosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alexandra Ehrens Benjamin Lenz Anna-Lena Neumann Samuela Giarrizzo Julia Jennifer Reichwald Stefan Julian Frohberger Wiebke Stamminger Benedikt Christian Buerfent Frédéric Fercoq Coralie Martin Daniel Kulke Achim Hoerauf Marc Peter Hübner |
spellingShingle |
Alexandra Ehrens Benjamin Lenz Anna-Lena Neumann Samuela Giarrizzo Julia Jennifer Reichwald Stefan Julian Frohberger Wiebke Stamminger Benedikt Christian Buerfent Frédéric Fercoq Coralie Martin Daniel Kulke Achim Hoerauf Marc Peter Hübner Microfilariae Trigger Eosinophil Extracellular DNA Traps in a Dectin-1-Dependent Manner Cell Reports filaria extracellular DNA traps eosinophils microfilaria L3 larva ETosis |
author_facet |
Alexandra Ehrens Benjamin Lenz Anna-Lena Neumann Samuela Giarrizzo Julia Jennifer Reichwald Stefan Julian Frohberger Wiebke Stamminger Benedikt Christian Buerfent Frédéric Fercoq Coralie Martin Daniel Kulke Achim Hoerauf Marc Peter Hübner |
author_sort |
Alexandra Ehrens |
title |
Microfilariae Trigger Eosinophil Extracellular DNA Traps in a Dectin-1-Dependent Manner |
title_short |
Microfilariae Trigger Eosinophil Extracellular DNA Traps in a Dectin-1-Dependent Manner |
title_full |
Microfilariae Trigger Eosinophil Extracellular DNA Traps in a Dectin-1-Dependent Manner |
title_fullStr |
Microfilariae Trigger Eosinophil Extracellular DNA Traps in a Dectin-1-Dependent Manner |
title_full_unstemmed |
Microfilariae Trigger Eosinophil Extracellular DNA Traps in a Dectin-1-Dependent Manner |
title_sort |
microfilariae trigger eosinophil extracellular dna traps in a dectin-1-dependent manner |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2021-01-01 |
description |
Summary: Eosinophils mediate protection against filarial nematodes. Our results demonstrate that eosinophil extracellular traps (EETosis) are induced by microfilariae and infective L3 larvae of Litomosoides sigmodontis. These extracellular DNA traps inhibit microfilariae motility in a DNA- and contact-dependent manner in vitro. Accordingly, microfilariae-injection triggers DNA release in an eosinophil-dependent manner in vivo and microfilariae covered with DNA traps are cleared more rapidly. Using dectin-1, we identify the required receptor for the microfilariae-induced EETosis, whereas signaling via other C-type lectin receptors, prior priming of eosinophils, and presence of antibodies are not required. The DNA released upon microfilariae-induced EETosis is mainly of mitochondrial origin, but acetylated and citrullinated histones are found within the traps. We further demonstrate that the presented DNA-dependent inhibition of microfilariae motility by eosinophils represents a conserved mechanism, as microfilariae from L. sigmodontis and the canine heartworm Dirofilaria immitis induce ETosis in murine and human eosinophils. |
topic |
filaria extracellular DNA traps eosinophils microfilaria L3 larva ETosis |
url |
http://www.sciencedirect.com/science/article/pii/S2211124720316107 |
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