ZOLA-3D allows flexible 3D localization microscopy over an adjustable axial range

3D single-molecule localization is limited in depth and often requires using a wide range of point spread functions (PSFs). Here the authors present an optical solution featuring a deformable mirror to generate different PSFs and easy-to-use software for super-resolution imaging up to 5 µm deep.

Bibliographic Details
Main Authors: Andrey Aristov, Benoit Lelandais, Elena Rensen, Christophe Zimmer
Format: Article
Language:English
Published: Nature Publishing Group 2018-06-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-04709-4