Monoclonal antibodies expression improvement in CHO cells by PiggyBac transposition regarding vectors ratios and design.

Monoclonal antibodies expression improvement in CHO cells by PiggyBac transposition regarding vectors ratios and design.

Establishing stable Chinese Hamster Ovary (CHO) cells producing monoclonal antibodies (mAbs) usually pass through the random integration of vectors to the cell genome, which is sensitive to gene silencing. One approach to overcome this issue is to target a highly transcribed region in the genome. Tr...

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Bibliographic Details
Main Authors: Samira Ahmadi, Fatemeh Davami, Noushin Davoudi, Fatemeh Nematpour, Maryam Ahmadi, Saeedeh Ebadat, Kayhan Azadmanesh, Farzaneh Barkhordari, Fereidoun Mahboudi
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5491063?pdf=render

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http://europepmc.org/articles/PMC5491063?pdf=render

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