Sequence-based prediction of permissive stretches for internal protein tagging and knockdown

Sequence-based prediction of permissive stretches for internal protein tagging and knockdown

Abstract Background Internal tagging of proteins by inserting small functional peptides into surface accessible permissive sites has proven to be an indispensable tool for basic and applied science. Permissive sites are typically identified by transposon mutagenesis on a case-by-case basis, limiting...

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Bibliographic Details
Main Authors: Sabine Oesterle, Tania Michelle Roberts, Lukas Andreas Widmer, Harun Mustafa, Sven Panke, Sonja Billerbeck
Format: Article
Language:English
Published: BMC 2017-10-01
Series:BMC Biology
Subjects:
Permissive site
Internal protein tagging
TEV protease
Protein knockdowns
Cell-free biotechnology
Online Access:http://link.springer.com/article/10.1186/s12915-017-0440-0

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http://link.springer.com/article/10.1186/s12915-017-0440-0

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